![]() Order this positive loading control protein now: Can be used for immuno-precipitation, affinity purification, Western blot and dot blot.Give you confidence in your Western blot protocol.Save time and resources for all your protein research projects.Perfect positive control protein for your Western blotting.Demonstrate that your protocol is efficient and correct.If you detect your protein of interest in the control lane, then an absence of the protein in other lanes is probably directly related to its low abundance rather than a faulty step in the blotting protocol. When trying to detect low-abundance proteins, it is especially important to know that your Western blot is functioning as expected. We strongly recommend the use of a positive control protein when setting up a new experiment this will give you immediate confidence in your Western Blot protocol. It also gives you greater confidence that the results in the other lanes are real rather than artifactual. It means all the steps of your Western blot functioned adequately, including gel electrophoresis, protein transfer to a blotting membrane, membrane blocking, and antibody labeling. Loading such protein into your positive control lane results in a reliably detectable protein sample. The multi-tag positive loading control protein is used to demonstrate that your Western Blot protocol is efficient and correct and that the antibody recognizes the target protein which may not be present in the experimental samples. The bound antibodies are then detected by developing the film. ![]() The membrane is then incubated with labels antibodies specific to the protein of interest. The gels are then transferred to a membrane producing a band for each protein. Researchers can identify specific proteins from a complex mixture of proteins extracted from cells using Western Blot.Ī mixture of proteins is separated based on molecular weight through gel electrophoresis. Western blotting is an important technique used in cell and molecular biology. ![]() Western Blot: Technique, Theory, and Trouble Shooting ![]()
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